这两个克隆都包含有一段DNA序列,能在在细胞周期蛋白d1的RNA被转录的时候将荧光蛋白绑定到其上。
Both clones included a DNA sequence that allow a fluorescent protein expressed in the cell to bind to cyclin D1 RNA the moment it is transcribed.
生物学中的许多现象如细胞凋亡、细胞周期调控、信号转导和癌变等均受蛋白质间相互作用的调控。
Many phenomena in biology, such as apoptosis, cell cycle control, signal transduction and canceration and etc, are all regulated by the interaction of proteins.
无细胞蛋白质合成系统相比传统的细胞表达系统有许多优点,包括表达周期短、反应条件容易控制等。
Cell-free protein synthesis has some advantages on protein expression and regulation, compared with traditional cell-based expression system.
热休克蛋白可能有助于细胞周期调控通过与原癌基因的产品或抑癌基因的产品。
Heat Shock Proteins may contribute to cell cycle regulation by interacting with proto-oncogene products or tumor suppressor gene products.
免疫组化法观察细胞周期调控蛋白的表达变化。
The expression of protein related cell cycle was observed by immunohistochemical.
容纳性细胞允许转录活化蛋白的转录和翻译,其激活病毒的早期基因和溶解性周期。
Permissive cells allow the transcription and translation of the transcriptional activator protein(ZEBRA), which activates the early genes of the virus and the lytic cycle.
衰老细胞呈现变革中的蛋白的表达调控细胞周期。
Senescent cells exhibit changes in the expression of proteins that regulate the cell cycle.
目的探索新克隆的编码人蛋白翻译起始因子的全长基因(C2基因)对细胞周期的影响及与细胞凋亡的关系。
Objective To investigate the effect of a new cloned full length gene, C2 gene which encodes a translation initiation factor, on cell cycle and the relationship between C2gene and apoptosis.
HR蛋白是细胞核受体辅阻遏物的发现证明HR蛋白是通过在毛发周期中调节基因表达来发挥作用的。
The discovery that the HR protein is a nuclear receptor corepressor indicated that HR function in hair cycling is by regulating gene expression.
正由于凋亡相关蛋白和细胞周期调节因子间的相互作用,使恶性肿瘤保持较高的细胞累积率。
It is because the reciprocal activation of apoptotic proteins and cell cycle control factors that maintain a higher cell accumulation rate in malignant tumors.
所以,鼠era蛋白在细胞周期中发挥重要作用。
So mouse ERA protein may play important role in eukaryote cell cycle.
结论缬沙坦可能通过影响细胞周期蛋白p 27表达而抑制肾脏肥大,从而发挥肾脏保护作用。
Conclusion the protective effect of valsartan on diabetic kidney may be partly through inhibiting renal hypertrophy by regulating the expression of cell cycle related protein P27.
背景:细胞周期调控蛋白水平及其相互关系是良恶性细胞增殖的主要区别标准。
BACKGROUND: the different level of proteins regulating cell cycle and their correlation is the main criteria to differentiate the benign and malignant cellular proliferation.
结论HCMV感染使部分细胞周期相关蛋白基因差异性表达可能是HCMV导致宿主细胞周期紊乱的原因之一。
Conclusions Significantly differential expression of some cell cycle related genes in infected cells could be the mechanism for the cell cycle dysregulation caused by HCMV infection.
目的观察dna甲基化和组蛋白乙酰化对人结肠癌细胞系癌相关基因的表达和细胞周期的影响。
Purpose to investigate the effects of DNA methylation and histone acetylation on the expression of tumor associated genes and cell cycle progression in human colonic cancer cells.
免疫细胞化学法检测细胞周期蛋白及凋亡相关基因蛋白的表达。
The expression of cell cycle protein and apoptosis-associated gene protein was detected with immunohistochemical technique.
目的:探讨苯那普利对糖尿病大鼠肾组织细胞周期蛋白激酶抑制剂p 21 CIP1蛋白表达的影响。
To investigate the effect of benazepril on expression of cyclin kinase inhibitor P21 CIP1 protein in renal tissue of diabetic rats.
细胞分裂周期运行和不同时相的转换通过CDC25磷酸酶去磷酸化和激活周期蛋白依赖性激酶(CDK)复合物来调控。
Cell Cycle Progression through the cell division cycle and transitions between various phases are regulated by using CDC25 phosphatases to activate specific cyclin-dependent kinase(CDK) complexes.
目的探讨经载体介导的RNA干扰对肝癌细胞周期蛋白E1表达抑制效率。
Objective To explore the inhibition of expression of cyclin E1 in hepatocellular carcinoma via RNA interference mediated by siRNA expressing vector.
方法利用免疫细胞化学,蛋白质印迹法,RT -PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。
Methods the cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry.
研究发现蛋白磷酸化参与细胞死亡途径的调节,影响亚细胞定位、细胞保护、细胞周期进程。
Protein phosphorylated participates in regulation of cell death pathway, location, cell protection, and transition of cell period.
目的探讨翼状胬肉组织中细胞周期蛋白依赖性激酶抑制剂P16的表达,了解其在翼状胬肉发病机制中的作用。
Objective To observe the expression of cyclin kinase inhibitor P16 in pterygium and to investigate its effects on the pathogenesis of pterygium.
目的筛选含有Tip30的涎腺腺样囊性癌高转移细胞,检测TIP30蛋白在涎腺腺样囊性癌高转移细胞中的表达,检测携带外源性TIP30细胞的细胞周期分布变化。
Objective To screen the adenoid cystic carcinoma-M with TIP30 and to detect the expression of TIP30 and cell cycle distribution by flow cytometry in ACC - M cells line.
细胞周期调控机制的核心是一组蛋白激酶。
The core of cell cycle regulation and control mechanism are relative to a group of protein kinase.
目的观察细胞周期蛋白依赖性激酶- 2 (CDK - 2)在SH -SY5Y细胞中的表达情况。
Objective To investigate the expression of cyclin-dependent kinase-2 (CDK-2) gene in SH-SY5Y cells.
结论5f能抑制体外微管蛋白聚合,从而可能是影响细胞的细胞周期及诱导凋亡途径之一。
Conclusion 5f inhibits tubulin polymerization and depolymerization, and leads to cell cycle arrested, and possibly induces apoptosis.
目的深入研究周期蛋白d 1在鼻咽癌细胞中的表达特征及其生物学功能。
Objective in the present study, we tried to further elucidate the expression and function of cyclin D1 in human nasopharyngeal carcinoma (NPC) cell line.
采用体外诱导卵母细胞成熟技术,比较研究了雌核发育银鲫和两性融合发育彩鲫卵母细胞成熟过程中周期蛋白合成和MPF活性变化情况。
The changes in cyclin synthesis and MPF activity during oocyte maturation in vitro in gynogenetic silver crucian carp and amphimictic colored crucian carp were compared.
采用体外诱导卵母细胞成熟技术,比较研究了雌核发育银鲫和两性融合发育彩鲫卵母细胞成熟过程中周期蛋白合成和MPF活性变化情况。
The changes in cyclin synthesis and MPF activity during oocyte maturation in vitro in gynogenetic silver crucian carp and amphimictic colored crucian carp were compared.
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